Ribose Studies

Ribose Study Summaries...

01. Ribose Improves Myocardial Function in Congestive Heart Failure

02. Ribose Maintains Ejection Fraction Following Aortic Valve Surgery

03. Effect of Ribose on the Detection of the Hibernating Myocardium During Low Dose Dobutine Stress Echocardiography

04. Effects of Ribose Supplementation on Adenine Nucleotide Concentration in Skeletal Muscle Following High-Intensity Exercise

05. Purine Salvage is Not Reduced During Recovery Following Intense Contractions

06. Effects of Ribose Supplementation on Performance During Repeated High-Intensity Cycle Sprints

07. The Effects of Creatine vs. Creatine Plus Ribose Supplementation on Anaerobic Working Capacity After Two Days of Loading

08. The Effect of Ribose on Immune Function

09. Ribose Supplementation Improves Muscular Strength and Endurance in Male Bodybuilders

10. More Scientific References

11. Ribose Information

01. Ribose Improves Myocardial Function in Congestive Heart Failure

Study Design: Double blind cross over, 3 weeks/arm with 1 week washout

Test Substances and Dose: ribose, dextrose (placebo) 5 grams / dose t.i.d.

Subjects: 15 adult patients with unstable coronary artery disease and CHF (12 reported)

Results: ECHO revealed a significant improvement in deceleration time of the E wave, stroke volume index, ejection fraction, atrial contribution and LV systolic volume.

Therapy	E Decel¹(msec)	SVI²-	EF²(%)	Ac¹(%)	LVVs²(ml)
Ribose	193.5 ± 45.9	2.63 ± 0.57	51.0 ± 7.3	45.3 ± 9.2	64.4 ± 24.8
Placebo	250.0 ± 70.2	1.99 ± 0.71	40.9 ± 14.2	39.2 ± 9.7	78.4 ± 27.0
¹P<0.05 ²P<0.01

Source: Illien, S, et al. University of Bonn, Bonn, Germany
For presentation to FASEB April 2001

02. Ribose Maintains Ejection Fraction Following Aortic Valve Surgery

Study Design: Double blind, post-surgical

Test Substances and Dose: 80mM ribose in D5W or D5W alone (placebo); IV; 5 days

Subjects: 20 adult patients with ejection fraction (EF) at least 35%; 10 in each group

Results: Eighty percent of the placebo patients demonstrated a decline at postoperative day 7 in EF of greater than 15% (range 15-56%) from baseline. Twenty percent of the ribose treated patients had a decline in EF at day 7 greater than 15%. No significant changes between either group or within each group was noted in altered myocardial chamber dimensions or hemodynamic parameters. This study suggests that the supplementation of ribose may prevent deterioration of left ventricular function peri-operatively in patients undergoing aortic valve surgery alone or in combination with coronary artery bypass graft.

Source: Illien, S, et al. University of Bonn, Bonn, Germany
For presentation to FASEB April 2001

03. Effect of Ribose on the Detection of the Hibernating Myocardium During Low Dose Dobutamine Stress Echocardiography

Study Design: 2 Day randomized, double blind cross over

Test Substances and Dose: Dextrose (placebo); ribose. Four hour infusion of test substance on first day with cross over on second day to alternate test substance.

Subjects: Twenty-five patients with ischemic cardiomyopathy (mean EF=30±8%). Eleven subjects underwent CABG following the test with follow-up echo obtained 1-3 months post-CABG.

Results: During DE wall motion improved in more dysfunctional segments on ribose compared to placebo (63 v 45, p=0.02). In patients undergoing CABG the sensitivity of DE+ribose for prediciton of functional recovery was 61% compared to 46% for DE+placebo (p=0.07). There was a positive relationship between changes in EF during DE+Ribose infusion and changes in EF after CABG (r=0.47) EF changes during DE+placebo infusion correlated negatively (r=-0.47). ribose increased the frequency of response to DE in hibernating regions with contractile dysfunction. The improvement in global systolic function with DE+ribose predicted the magnitude of improvement achieved with revascularization.

Source: Sawada,S, I Pizlo. Indiana University School of Medicine; Indianapolis, Indiana; circulation 100(18):3394, 1999; Partial completion of Phase II clinical trial.

04. Effects of Ribose Supplementation on Adenine Nucleotide Concentration in Skeletal Muscle Following High-Intensity Exercise

Study Design: Eleven day double blind, placebo controlled trial including three days of pre-test supplementation, five days of high-intensity exercise (two bouts / day) and 65 hours of rest. Muscle biopsies obtained pre-supplementation, pre-exercise, post-exercise and following rest.

Test Substances and Dose: Dextrose (placebo); ribose 10 gram dose b.i.d. (20 gram / day)

Subjects: Sixteen healthy male volunteers

Results: Ribose supplementation attenuated decrease in total adenine nucleotides (TAN) observed during exercise (p<0.05). Following rest, ribose subject TAN levels returned to baseline, while placebo subjects remained depressed 23% (p<0.05). Ribose maybe beneficial in the short-term replenishment of TAN immediately following a high-intensity exercise bout.

Source: Gallagher, PM, et al. Ball State University; Muncie, Indiana; Presented at ACSM, October 2000

05. Purine Salvage is Not Reduced During Recovery Following Intense Contractions

Study Design: Salvage of 2.0 mM [3H]-adenine to ATP was compared in the presence or absence of 5 mM ribose during cell-free perfusion with Krebs-Henseleit buffer in an isolated rat hindlimb preparation (n=5/group) following intense contraction and 1 hour rest.

Results: Return of [ATP] was incomplete, as the reamination of IMP to AMP/ATP was only 75-80% complete. Adenine salvage rates in resting and recovering muscle were relatively low, but increasesd approximately five-fold in the presence of ribose.

Conclusion:The high rate of adenine salvage with ribose could be important in ATP recovery of intense contractions, especially if IMP were further degraded to inosine and subsequently lost from the cell.

Source: Zarzeczny, R, et al. University of Missouri; Columbia, Missouri Medicine & Science in Sports & Exercise, 32(5):Supplement, May 2000

06. Effects of Ribose Supplementation on Performance During Repeated High-Intensity Cycle Sprints

Study Design: Eleven day double blind, placebo controlled trial including three days of pre-test supplementation, five days of high-intensity exercise (2 bouts / day) and 65 hours of rest. Exercise bouts consisted of ten sessions consisting of 15 X 10 second sprints at 7% body weight. Power output and fatigue were measured.

Test Substances and Dose: Dextrose (placebo); ribose 10 gram dose b.i.d. (20 gram / day)

Subjects: Sixteen healthy male volunteers

Results: The ribose group had a greater change in mean power output during the training period than the placebo group (4.2% v 0.6%) and a greater change in peak power output between the last sprint session than the first (a measure of training effect; p<0.05). There was no statistically significant difference in fatigue index. The data suggest that ribose supplementation may provide an ergogenic benefit over time with high-intensity exercise.

Source: Gallagher, PM, et al. Ball State University; Muncie, Indiana; Presented at ACSM, October 2000

07. The Effects of Creatine vs Creatine plus Ribose Supplementation on Anaerobic Working Capacity After Two Days of Loading

Study Design:Placebo controlled, double blind

Test Substances and Dose: Dextrose (placebo), creatine, ribose, creatine + ribose; 5 gram / dose q.i.d.

Subjects: Thirty-eight helathy men and women 10/group; 8 ribose alone)

Change in Anaerobic Working Capacity
Placebo	7% change
Creatine	18% change
Ribose	29% change
Cr + Ribose	37% change

Source: Stout, J. Creighton University, Omaha, Nebraska

08. The Effect of Ribose on Immune Function

Study Design: HL-60 cells, induced to differentiate with retinoic acid, were incubated with no ribose or with ribose in concentrations of 1.0 to 50.0 mm. Respiratory burst was determined following four days of incubation.

Results: As the concentration of ribose in the differentiating cultures increased, the respiratory burst increased significantly. Cultures containing 5 and 10 mm / L ribose had significantly greater production of superoxide anion than control. Cultures with 25 and 50 mm / L ribose were no longer viable. Cells incubated with 10 mm / L glucose did not have an increase in respiratory burst.

Conclusion: An increased respiratory burst suggests that the population is more differentiate when incubated with ribose than without. (Manuscript in preparation).

Source: Percival, S. University of Florida; Gainseville, Florida; Presented at FASEB, April 2000

09. Ribose Supplementation Improves Muscular Strength and Endurance in Male Bodybuilders

Study Design: Double blind placebo controlled, four weeks on test substance

Test Substances and Dose: ribose, dextrose (placebo) 5 grams / dose b.i.d.

Subjects: Twenty young, healthy male bodybuilders

Results: Ribose supplementation resulted in a significant increase in the number of total repetitions performed for ten sets in the bench press as well as an increase in maximal strength.

Treatment	1-RM Pre(Kg)	1-RM Post(Kg)	Total Reps Pre(100% BW)	Total Reps Post(100% BW)
Ribose	114.1 ± 13.6	117.7 ± 14.0¹	24.5 ± 7.6	29.3 ± 7.5²(19.6% Inc.)
Placebo	129.6 ± 14.2	131.8 ± 14.5	34.1 ± 8.6	38.2 ± 8.0(12.0% Inc.)
¹P=0.01 ²P=0.03

Source: Antonio, J., et al. University of Delaware, Newark, DE; For presentation to FASEB April 2001

Essense-of-Life, LLC is NOT ASSOCIATED in any way with the Eniva Corporation, Rainbow Minerals, Wolf Clinic or Nutrition 2000. (...more)

DISCLAIMER: The information contained herein is not medical advice and is not intended to replace the advice or attention of your personal physician (or your pet's veterinarian) or other health care professionals. You must consult your health care provider (or your pet's veterinarian) before beginning any new dietary supplementation program. This information is not intended as a "prescription" for treatment nor is it intended to diagnose, treat, cure or prevent any disease. Essense-of-life.com does not suggest, endorse, or imply in any way any treatment or cure for any ailment or disease nor does Essense-of-life.com endorse or suggest that you should ever take more than the recommended dose of any nutritional supplement as listed on the label. Essense-of-life.com makes no representations concerning the efficacy, appropriateness, or suitability of any products or treatments. Neither Essense-of-life.com nor any other party involved in providing this Web site are doctors and have no medical background or training. In view of the possibility of human error, no party involved in providing this web site warrants that the information contained herein is in any respect accurate or complete and they are not responsible nor liable for any errors or omissions that may be found in this web site or for the results obtained from the use of such information. The information on this site is for educational purposes only. If you (or your pet) are ill, see a health care professional. Products (or their distributors) mentioned on this site do not make any claim to any specific benefits which might be achieved by using them. This information is not specific to any company's products. Statements have not been evaluated by the U.S. Food and Drug Administration. The entire risk as to use of this web site is assumed by the user.

RIBOSE STUDIES